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KMID : 0382419980240030099
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Korean Journal of Environmental Health Society
1998 Volume.24 No. 3 p.99 ~ p.106
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Determination of Short-term Bioconcentration Factor on Dichlorvos , Methidathion and Phosalone in Brachydanio rerio and Xiphophorus Hellieri
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Min Kyung-Jin
Cha Chun-Geun
Jeon Bong-Sik
Kim Keun-Bae
Cho Young-Joo
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Abstract
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This study was performed to investigate the bioconcentration of dichlorvos, methidathion and phosalone in zebrafish(brachydanio rerio), red sword tail (Xiphophorus hellieri). The fishes were exposed to 0.05 ppm, 0.01 ppm, 0.50 ppm, one-hundredth concentration of 96-hrs LC_50 and one-thousandth concentration of 96-hrs LC_50 and test periods were 3, 5 and 8 days. The depuration rate of each pesticide from the whole body of fish was determined over the 24-hr period after treatment. Obtained results are summerized as follows: In the case of dichlorvos, dichlorvos concentration in zebrafish extract and BCF_s of dichlorvos were increased as increasing test concentration. In the case of same experimental concentrations, dichlorvos concentration in zebrafish extract and BCF_s of dichlorvos were decreased as prolonging test periods, especially dropped after 5days. Dichlorvos concentration in red sword tail extract were increased as increasing test concentration, but BCF_s in concentration of 0.05 ppm, 0.01 ppm and one-hundredth of 96-hrs LC_50 were decreased. Methidathion and phosalone concentration in zebrafish extract in zebrafish extract were increased as increasing test concentration, but there was little difference in BCF_s. In the case of same experimental concentrations, there were little differences in BCF_s and concentration in zebrafish extract. In the case of red sword tail, it was impossible to calculate on BCF_s data because test concentration was under the detecting limit on GC or test fish were die. Determined depuration rate conatant were highest on dichlorvos, and followed by methidathion, and phosalone. The results of determining depuration rate of these pesticides showed that the high BCF in fish might be due to the slow depuration rate in fish, it is thought to be responsible for vapor pressure, water solubility and partition coefficient. It is suggested that one-hundredth concentration of 96-hrs LC_50 will be proper test concentration because one-thousundth of LC_50 was under the detecting limit on GC. Dichlorvos, methidathion and phosalone, organophosphorous pesticides, were examined to their BCF_s and depuration rates by means of fish test.
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